Crest Optics DeepSIM
Super resolution microscopy that answers the deep biological questions effortlessly
For the advancement of science, we at CrestOptics think that super-resolution should be available to scientists everywhere. DeepSIM is the first super-resolution module designed to work with any existing upright or inverted microscope that has a camera connection. A confocal microscope-like interface makes it simple for scientists to gather detailed information from biological samples.
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Confocal Upgrade
Stand-Alone Super Resolution
Deep Imaging
~100nm Resolution in XY
DESCRIPTION
Reliable, Simple to Use and Affordable Solution
Based on a multi-spot structured illumination system, the DeepSIM is a reliable, simple to use and affordable solution to study cellular structures up to an XY resolution of ~100nm.
Super-resolved optical sectioning, with Z resolution up to ~ 300nm, can be obtained using both high (60X – 100X) and low magnification (20x – 40x) objectives to expand the range of applications to include complex 3D models such as tissues, organoids, spheroids and small organisms.
DEEPER
IMAGING
Super-Resolved Data Over 50µm Z in Depth
The DeepSIM is designed to work with samples of thicknesses comparable to those used in confocal microscopy, giving super-resolved data over 50µm Z in depth in non-clarified samples.
This means that more meaningful data can be obtained from native heterogeneous complex samples using routine preparation protocols.
DeepSIM enables the effortless study of live-cell dynamics through a temporal resolution greater than 10fps (1024×1024 px FOV), allowing biological changes to be tracked at cellular and subcellular levels.
What You Can See
How You See
WIDEFIELD
CONFOCAL
DeepSIM SUPER RESOLUTION
EVOLUTION THROUGH COMPATIBILITY
DeepSIM is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system available either as Super Resolution add-on module for CrestOptics X-light V3 Spinning Disk system or standalone equipment.
TECHNICAL SPECIFICATIONS
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FOV: 1024x1024pixel (66x66µm 100X | 333x333µm 20X)
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Laser: Spectral range: excitation: 400-750 nm; emission: 400-850 nm
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Camera: Compatible with any triggerable camera having 6.5 µm pixel size
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Dual camera option: Available
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Acquisition speed: 13fps (1024x1024px)
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Spinning disk upgrade: Compatible with CrestOptics X-light V3
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Objectives compatible: From 100X to 20X range
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Resolution: Lateral Resolution (FWHM): ~100 nm (100X NA 1.45) Axial Resolution (FWHM): ~300 nm (100X NA 1.45)
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Software: Micro-Manager / VisiView® / NIS elements
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Recommended Installation Conditions: Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation)
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Weight: 44 lbs | 20Kg
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Dimensions: 14.0 (w) x 17.1 (L) x 11.8 (h) inches | 356.0 (w) x 435.0 (L) x 300.0 (h) mm
HIGH END IMAGING
AT AN AFFORDABLE
PRICE
Confocal/Super Resolution/TIRF Packages Available using industry-leading products
Voxyl Offers a variety of confocal systems. These systems are configured based on your exact needs and budget. Please contact Voxyl for more information on our current confocal and TIRF systems
